Speedy™ Mouse AFP One-Step ELISA Kit
Sensitivity
15.5 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Cat no : SE60008
Synonyms
alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse AFP One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 15.5 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11576 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 106% | 93%-124% |
| Cell culture supernatant | 104% | 94%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,555.0 | 62.6 | 2.5 |
| 2 | 8 | 657.2 | 13.8 | 2.1 |
| 3 | 8 | 209.7 | 5.0 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,522.1 | 61.3 | 2.4 |
| 2 | 16 | 661.4 | 33.2 | 5.0 |
| 3 | 16 | 209.1 | 4.5 | 2.2 |
Background Information
AFP (alpha-fetoprotein) is a major plasma protein found in the fetus while plasma AFP level is decreased rapidly after birth. AFP is a vital marker of the hepatocyte lineage. High AFP concentrations is related with tumor cell growth. Detection of AFP in plasma is important in diagnosis of hepatocellular carcinoma (HCC), stomach cancer and germ cell cancers. Altered levels of both fetal and maternal AFP are associated with hypothyroidism, autoimmune disorders, and heart defects. Measurement of AFP in maternal blood or amniotic fluid of pregnant women is used in screening developmental abnormalities such as aneuploidy. Serum AFP level is elevated in people with developmental birth defects and neurologic disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein |