Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells, mouse liver tissue |
| Positive IF/ICC detected in | MCF-7 cells, NIH/3T3 cells, HeLa cells |
| Positive Dot Blot detected in | / / |
| Positive ChIP-qPCR detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86990-1-RR targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 1, H3a |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_3745259 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 86990-1-RR | Download protocol |
| WB protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 86990-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |