Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
83768-3-RR targets Mmp9 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Fusion Protein 相同性解析による交差性が予測される生物種 |
| Full Name | matrix metallopeptidase 9 |
| Calculated molecular weight | 79kd |
| Observed molecular weight | 92 kDa |
| GenBank accession number | NM_031055.2 |
| Gene Symbol | Mmp9 |
| Gene ID (NCBI) | 81687 |
| RRID | AB_3671362 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P50282 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mmp9 (Matrix metalloproteinase-9) belongs to matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. The immunogen sequence of this antibody is of rat origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Mmp9 antibody 83768-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Oncol Tumor-stroma proportion is associated with increased M2 macrophage abundance and predicts the resistance to immune checkpoint blockade in breast cancer | ||
Toxicon Mechanistic insights into PI3K/Akt-MMP9 signaling in Crotonis Fructus-induced reproductive toxicity via integrated network toxicology and multi-model validation. | ||
Cell Signal DOT1L promotes phenotypic transition of vascular smooth muscle cells following vascular injury in diabetes through activation of the FGFR4/PI3K/AKT/Jag/Notch signaling pathway. |