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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50079-1

    Sandwich ELISA standard curve of MP50079-1

    Sandwich ELISA standard curve of MP50079-1, Rat Mmp9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68695-1-PBS. Detection antibody: 68695-3-PBS. Standard: Eg0439. Range: 62.5-4000 pg/mL.

  • Sample test of MP50079-1

    Sample test of MP50079-1

    Rat spleen and lung tissues from one adult rat (chopped in 1-2 mm pieces in 30 mL of medium) were cultured in RPMI supplemented with 10% fetal bovine serum and 50 μM β-mercaptoethanol. Aliquots of the cell culture supernates were removed, assayed for levels of rat MMP-9, and measured 5,145.7 pg/mL and 540.2 pg/mL, respectively.

  • Sample test of MP50079-1

    Sample test of MP50079-1

    Serum of sixteen rats was measured. The MMP-9 concentration of detected samples was determined to be 37.0 ng/mL with a range of 22.7-48.2 ng/mL.

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Rat Mmp9 Monoclonal antibody, PBS Only (Capture)

Mmp9 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68695-1-PBS
Clone No.1B1C2

Host / Isotype

Mouse / IgG1

Reactivity

rat

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

matrix metallopeptidase 9, Matrix metalloproteinase-9

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68695-1-PBS targets Mmp9 as part of a matched antibody pair:

MP50079-1: 68695-1-PBS capture and 68695-3-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Mmp9 fusion protein Eg0439 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 9
Calculated molecular weight79kd
GenBank accession numberNM_031055.2
Gene Symbol Mmp9
Gene ID (NCBI) 81687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.