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Validation Data Gallery

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  • Western Blot (WB) analysis of TF-1 cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of TF-1 using 67593-1-Ig (same clone as 67593-1-PBS)

    TF-1 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of human placenta using 67593-1-Ig (same clone as 67593-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of human spleen using 67593-1-Ig (same clone as 67593-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of K-562 using 67593-1-Ig (same clone as 67593-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of LNCaP using 67593-1-Ig (same clone as 67593-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of L02 using 67593-1-Ig (same clone as 67593-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Mitoferrin 1 Monoclonal antibody (67593-1-Ig)

    WB analysis of HepG2 using 67593-1-Ig (same clone as 67593-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67593-1-Ig (Mitoferrin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67593-1-PBS in a different storage buffer formulation.

Mitoferrin 1 Monoclonal antibody, PBS Only

Mitoferrin 1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67593-1-PBS
Clone No.1B1B2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

HT015, MFRN, Mitoferrin 1, MSC, MSCP, PRO1278, PRO1584, PRO2217, SLC25A37

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67593-1-PBS targets Mitoferrin 1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26216

Product name: Recombinant human SLC25A37 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-44 aa of BC132799

Sequence: MELRSGSVGSQAVARRMDGDSRDGGGGKDATGSEDYENLPTSAS

相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 37
Observed molecular weight 40 kDa
GenBank accession numberBC132799
Gene Symbol Mitoferrin 1
Gene ID (NCBI) 51312
RRIDAB_2882800
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9NYZ2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitoferrin (mfrn, SLC25A37), which is highly expressed in fetal and adult hematopoietic tissues, is a member of the vertebrate mitochondrial solute carrier family (SLC25) and is located on the mitochondrial inner membrane. It functions as an essential and high-affinity iron importer for the biosynthesis of mitochondrial heme and iron-sulfur cluster in vertebrate erythroblasts. Additionally, since it was identified as a major depressive disorder (MDD) risk gene, it is likely to be used as a potential biomarker of MDD diagnose.


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