Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, mouse brain tissue, rat liver tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84879-5-RR targets Methylmalonyl Coenzyme A mutase/MUT in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10523 Product name: Recombinant human MUT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 401-750 aa of BC016282 Sequence: SARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDTVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGVSTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV 相同性解析による交差性が予測される生物種 |
| Full Name | methylmalonyl Coenzyme A mutase |
| Calculated molecular weight | 750 aa, 83 kDa |
| Observed molecular weight | 78 kDa |
| GenBank accession number | BC016282 |
| Gene Symbol | MUT |
| Gene ID (NCBI) | 4594 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P22033 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 84879-5-RR | Download protocol |
| IHC protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 84879-5-RR | Download protocol |
| WB protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 84879-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |