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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Mesothelin Monoclonal antibody (66404-1-Ig)

    WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Mesothelin Monoclonal antibody (66404-1-Ig)

    WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Mesothelin Monoclonal antibody (66404-1-Ig)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Mesothelin Monoclonal antibody (66404-1-Ig)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

Mesothelin Monoclonal antibody, PBS Only

Mesothelin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66404-1-PBS
Clone No.2E3A4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66404-1-PBS targets Mesothelin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag23606

Product name: Recombinant human MSLN protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 273-621 aa of BC003512

Sequence: RSSRDPSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSVQEALSGTPCLLGPGPVLTVLALLLASTLA

相同性解析による交差性が予測される生物種
Full Name mesothelin
Calculated molecular weight 69 kDa
Observed molecular weight 69 kDa, 40 kDa
GenBank accession numberBC003512
Gene Symbol MSLN
Gene ID (NCBI) 10232
RRIDAB_2881778
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ13421
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).