• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MLPH Monoclonal antibody (66092-1-Ig)

    WB analysis using 66092-1-Ig (same clone as 66092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using MLPH Monoclonal antibody (66092-1-Ig)

    WB analysis of pig brain using 66092-1-Ig (same clone as 66092-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MLPH Monoclonal antibody (66092-1-Ig)

    WB analysis of HEK-293 using 66092-1-Ig (same clone as 66092-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using MLPH Monoclonal antibody (66092-1-Ig)

    WB analysis of PC-3 using 66092-1-Ig (same clone as 66092-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MLPH Monoclonal antibody (66092-1-Ig)

    IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLPH Monoclonal antibody (66092-1-Ig)

    IF Staining of HepG2 using 66092-1-Ig (same clone as 66092-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66092-1-Ig (Melanophilin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

MLPH Monoclonal antibody, PBS Only

MLPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66092-1-PBS
Clone No.1A8D12

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A, SLAC2A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66092-1-PBS targets MLPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19286

Product name: Recombinant human MLPH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 8-236 aa of BC001653

Sequence: SKLTDEEAQHVLEVVQRDFDLRRKEEERLEALKGKIKKESSKRELLSDTAHLNETHCARCLQPYQLLVNSKRQCLECGLFTCKSCGRVHPEEQGWICDPCHLARVVKIGSLEWYYEHVKARFKRFGSAKVIRSLHGRLQGGAGPELISEERSGDSDQTDEDGEPGSEAQAQAQPFGSKKKRLLSVHDFDFEGDSDDSTQPQGHSLHLSSVPEARDSPQSLTDESCSEKA

相同性解析による交差性が予測される生物種
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
RRIDAB_11232039
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BV36
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.