Validation Data Gallery
Tested Applications
| Positive WB detected in | SK-MEL-30 cells |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87351-4-RR targets Melan-A in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13346 Product name: Recombinant human MLANA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-118 aa of BC014423 Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP 相同性解析による交差性が予測される生物種 |
| Full Name | melan-A |
| Calculated molecular weight | 13 kDa |
| Observed molecular weight | 13-20 kDa |
| GenBank accession number | BC014423 |
| Gene Symbol | MelanA |
| Gene ID (NCBI) | 2315 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16655 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Melan-A antibody 87351-4-RR | Download protocol |
| WB protocol for Melan-A antibody 87351-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |