Product Information

Proteintech's PR30004 (Mayer's Hematoxylin Solution) can be used for tissue and cell staining. Mayer's Hematoxylin can stain the cell nucleus to produce a blue color. The formula of this product has been optimized to avoid using toxic chemicals, e.g., mercury or methanol. Differentiation and bluing are not needed. The staining is simple and time-saving. 

The product is provided in a ready-to-use format. No further dilution is required. Each milliliter of this product can stain ~10 slides.

Storage

Ambient. Stable for one year.

Usage

IHC counter staining:  

After immunohistochemistry signal development, apply the product to the tissue slides to make sure the tissues are fully covered. After staining for 1-2 minutes, rinse the slides thoroughly with TBS bluing buffer for 1-2 minutes, followed by immersing in distilled water for 2 minutes, or rinse the slides in distilled water, followed by immersing in a saturated Na₂HPO₄ solution for 2 minutes. Once the slides turn blue, they can be washed, dehydrated, cleared, mounted, and observed.

H&E staining: 

1. For FFPE samples: Immerse slides in xylene for 10 minutes. Repeat in fresh xylene again for another 10 minutes. Then rehydrate the slides by sequentially incubating in 100%, 95%, 80%, and 60% ethanol solutions for 5 minutes each. Finally, wash the slides with distilled water for 2 minutes.

2. Apply the product onto the tissue slides and make sure the tissues are fully covered. After staining for 1-2 minutes, rinse the slides thoroughly with TBST bluing buffer for 1-2 minutes followed by immersing in distilled water for 2 minutes, or rinse the slides in distilled water followed by immersing in a saturated Na₂HPO₄ solution for 2 minutes. Apply Eosin staining then. 

3. For frozen samples: Wash the samples with distilled water. Then, perform staining using the same protocol as above. 

4. For cell samples: Fix the cells with 4% PFA for 10 minutes or longer. After fixation, wash the cells twice with distilled water for 2 minutes. Then, stain with the same protocol as above. If the cells are embedded with paraffin, please de-paraffin using the FFPE sample protocol above.

Cautions

1. Stain with 1-2 samples to make sure the staining achieves optimal results. 

2. Wear a lab coat and gloves during operation.

Cited in Article as

pr30004, Mayer's Hematoxylin Solution, Proteintech, IL, USA