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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Matrilin 2 Polyclonal antibody (24064-1-AP)

    WB analysis of NIH/3T3 using 24064-1-AP (same clone as 24064-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using Matrilin 2 Polyclonal antibody (24064-1-AP)

    WB analysis of mouse liver using 24064-1-AP (same clone as 24064-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Matrilin 2 Polyclonal antibody (24064-1-AP)

    WB analysis of HEK-293 using 24064-1-AP (same clone as 24064-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Matrilin 2 Polyclonal antibody (24064-1-AP)

    IHC staining of mouse kidney using 24064-1-AP (same clone as 24064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24064-1-AP (Matrilin 2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Matrilin 2 Polyclonal antibody (24064-1-AP)

    IHC staining of mouse kidney using 24064-1-AP (same clone as 24064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24064-1-AP (Matrilin 2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Matrilin 2 Polyclonal antibody (24064-1-AP)

    IHC staining of mouse kidney using 24064-1-AP (same clone as 24064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24064-1-AP (Matrilin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24064-1-PBS in a different storage buffer formulation.

Matrilin 2 Polyclonal antibody, PBS Only

Matrilin 2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24064-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

MATN2, Matrilin-2, UNQ193/PRO219

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24064-1-PBS targets Matrilin 2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21113

Product name: Recombinant human MATN2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 26-351 aa of BC010444

Sequence: RSRGRSISRGRHARTHPQTALLESSCENKRADLVFIIDSSRSVNTHDYAKVKEFIVDILQFLDIGPDVTRVGLLQYGSTVKNEFSLKTFKRKSEVERAVKRMRHLSTGTMTGLAIQYALNIAFSEAEGARPLRENVPRVIMIVTDGRPQDSVAEVAAKARDTGILIFAIGVGQVDFNTLKSIGSEPHEDHVFLVANFSQIETLTSVFQKKLCTAHMCSTLEHNCAHFCINIPGSYVCRCKQGYILNSDQTTCRIQDLCAMEDHNCEQLCVNVPGSFVCQCYSGYALAEDGKRCVAVDYCASENHGCEHECVNADGSYLCQCHEGFA

相同性解析による交差性が予測される生物種
Full Name matrilin 2
Calculated molecular weight 107 kDa
Observed molecular weight 120-150 kDa
GenBank accession numberBC010444
Gene Symbol Matrilin 2
Gene ID (NCBI) 4147
RRIDAB_2879422
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDO00339
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The matrilins represent a new family of oligomeric non-collagenous extracellular matrix proteins that are assumed to act as adapter molecules connecting other proteins and proteoglycans in the extracellular matrix (PMID: 17513098). Four matrilins (matrilin-1, -2, -3, and -4) have been identified. They all share a structure made up of von Willebrand factor A domains, EGF-like domains and a coiled-coil alpha-helical module (PMID: 18386166). Matrilin-1 and matrilin-3 are expressed mainly in cartilage, while matrilin-2 and matrilin-4 show broader tissue distribution. Matrilin-2 is the largest member of the family. It incorporates into loose and dense connective tissue and becomes associated with some basement membranes (PMID: 17513098).

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