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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Maspin Polyclonal antibody (11722-1-AP)

    WB analysis of HepG2 using 11722-1-AP (same clone as 11722-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Maspin Polyclonal antibody (11722-1-AP)

    WB analysis of HeLa using 11722-1-AP (same clone as 11722-1-PBS)

    WB result of Maspin antibody (11722-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Maspin transfected HeLa cells. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Maspin Polyclonal antibody (11722-1-AP)

    WB analysis of A431 using 11722-1-AP (same clone as 11722-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Maspin Polyclonal antibody (11722-1-AP)

    WB analysis of HeLa using 11722-1-AP (same clone as 11722-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human lung cancer using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human bowen disease using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11722-1-AP (Maspin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human bowen disease using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11722-1-AP (Maspin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human pancreas using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human pancreas using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human breast cancer using 11722-1-AP (same clone as 11722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11722-1-AP (Maspin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Maspin Polyclonal antibody (11722-1-AP)

    IF Staining of A431 using 11722-1-AP (same clone as 11722-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Maspin antibody (11722-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Maspin Polyclonal antibody, PBS Only

Maspin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11722-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SERPINB5, Peptidase inhibitor 5, PI 5, PI5, PI-5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11722-1-PBS targets Maspin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2304

Product name: Recombinant human SERPINB5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-231 aa of BC020713

Sequence: MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRVNKVCGAACSSKRSPIIDVKNDRDRVGHKSIPMRNLRARPAKCLS

相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 5
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC020713
Gene Symbol Maspin
Gene ID (NCBI) 5268
RRIDAB_10597111
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP36952
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SERPINB5, also named PI-5, Maspin, and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.

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