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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYST3 Recombinant monoclonal antibody (86963-1-RR)

    WB analysis using 86963-1-RR (same clone as 86963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86963-1-RR (MYST3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86963-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MYST3 Recombinant monoclonal antibody (86963-1-RR)

    IF Staining of MCF-7 using 86963-1-RR (same clone as 86963-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MYST3 antibody (86963-1-RR, Clone: 251927F2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86963-1-PBS in a different storage buffer formulation.

  • Affinity of 86963-1-RR

    Affinity of 86963-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86963-1-RR against Human MYST3 were performed. The affinity constant is 0.275 nM.

MYST3 Recombinant monoclonal antibody, PBS Only

MYST3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86963-1-PBS
Clone No.251927F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KAT6A, MOZ, MYST 3, RUNXBP2, Zinc finger protein 220

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86963-1-PBS targets MYST3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag29729

Product name: Recombinant human MYST3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 420-510 aa of BC142659

Sequence: SPDGRKARGEVVDYSEQYRIRKRGNRKSSTSDWPTDNQDGWDGKQENEERLFGSQEIMTEKDMELFRDIQEQALQKVGVTGPPDPQVRCPS

相同性解析による交差性が予測される生物種
Full Name MYST histone acetyltransferase (monocytic leukemia) 3
Calculated molecular weight 2004 aa, 225 kDa
Observed molecular weight310 kDa
GenBank accession numberBC142659
Gene Symbol MYST3
Gene ID (NCBI) 7994
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ92794
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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