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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYPN-Specific Polyclonal antibody (16180-1-AP)

    WB analysis using 16180-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MYPN-Specific Polyclonal antibody (16180-1-AP)

    WB analysis of HeLa using 16180-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MYPN-Specific Polyclonal antibody (16180-1-AP)

    WB analysis of HeLa using 16180-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MYPN-Specific Polyclonal antibody (16180-1-AP)

    WB analysis of HeLa using 16180-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MYPN-Specific Polyclonal antibody (16180-1-AP)

    WB analysis of HeLa using 16180-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MYPN-Specific Polyclonal antibody (16180-1-AP)

    WB analysis of HeLa using 16180-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYPN-Specific Polyclonal antibody (16180-1-AP)

    IHC staining of mouse skeletal muscle using 16180-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYPN-Specific Polyclonal antibody (16180-1-AP)

    IHC staining of mouse heart using 16180-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYPN-Specific Polyclonal antibody (16180-1-AP)

    IHC staining of human skeletal muscle using 16180-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYPN-Specific Polyclonal antibody (16180-1-AP)

    IHC staining of human skeletal muscle using 16180-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYPN-Specific Polyclonal antibody (16180-1-AP)

    IHC staining of human heart using 16180-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYPN-Specific Polyclonal antibody (16180-1-AP)

    IHC staining of human heart using 16180-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYPN-Specific Polyclonal antibody (16180-1-AP)

    IF Staining of HepG2 using 16180-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYPN-Specific antibody (16180-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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MYPN-Specific Polyclonal antibody

MYPN-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16180-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MYPN, Myopalladin, MYOP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inmouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16180-1-AP targets MYPN-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myopalladin
Calculated molecular weight 145 kDa
Observed molecular weight 145 kDa
GenBank accession numberNM_032578
Gene Symbol MYPN
Gene ID (NCBI) 84665
RRIDAB_2146767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TC9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin.

Protocols

Product Specific Protocols
WB protocol for MYPN-Specific antibody 16180-1-APDownload protocol
IHC protocol for MYPN-Specific antibody 16180-1-APDownload protocol
IF protocol for MYPN-Specific antibody 16180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.

Authors - Hexiao Shen
View Article