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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    WB analysis using 20360-1-AP (same clone as 20360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    WB analysis of mouse skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IHC staining of human skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IHC staining of human skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IHC staining of human heart using 20360-1-AP (same clone as 20360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IHC staining of human heart using 20360-1-AP (same clone as 20360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IHC staining of human heart using 20360-1-AP (same clone as 20360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IHC staining of human heart using 20360-1-AP (same clone as 20360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IF Staining of mouse skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IF Staining of mouse skeletal muscle using 20360-1-AP (same clone as 20360-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IF Staining of mouse heart using 20360-1-AP (same clone as 20360-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IF Staining of mouse heart using 20360-1-AP (same clone as 20360-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using MYOM1-Specific Polyclonal antibody (20360-1-AP)

    IF Staining of C2C12 using 20360-1-AP (same clone as 20360-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYOM1-Specific antibody (20360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20360-1-PBS in a different storage buffer formulation.

MYOM1-Specific Polyclonal antibody, PBS Only

MYOM1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 20360-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20360-1-PBS targets MYOM1-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name myomesin 1, 185kDa
Calculated molecular weight 188 kDa
Observed molecular weight 190 kDa
GenBank accession numberNM_003803
Gene Symbol MYOM1
Gene ID (NCBI) 8736
RRIDAB_10696312
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP52179
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent. The antibody is specific to MYOM1.

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