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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using MYOF Recombinant antibody (82917-1-RR)

    FC experiment of HepG2 using 82917-1-RR (same clone as 82917-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human MYOF (82917-1-RR, Clone:230213C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82917-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MYOF Recombinant antibody (82917-1-RR)

    FC experiment of HeLa using 82917-1-RR (same clone as 82917-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MYOF (82917-1-RR, Clone:230213C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82917-1-PBS in a different storage buffer formulation.

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MYOF Recombinant antibody, PBS Only

MYOF Uni-rAbTM Recombinant Antibody for FC (Intra), Indirect ELISA
Cat No. 82917-1-PBS
Clone No.230213C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Indirect ELISA

Synonyms

Myoferlin, KIAA1207, Fer-1-like protein 3, FER1L3, Fer 1 like protein 3

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82917-1-PBS targets MYOF in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myoferlin
Calculated molecular weight 235 kDa
GenBank accession numberNM_133337
Gene Symbol MYOF
Gene ID (NCBI) 26509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZM1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYOF, also named FER1L3 and KIAA1207, belongs to the Ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR.