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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYOF Polyclonal antibody (19548-1-AP)

    WB analysis using 19548-1-AP (same clone as 19548-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19548-1-AP (MYOF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MYOF Polyclonal antibody (19548-1-AP)

    WB analysis of A549 using 19548-1-AP (same clone as 19548-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using MYOF Polyclonal antibody (19548-1-AP)

    WB analysis of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYOF Polyclonal antibody (19548-1-AP)

    IHC staining of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYOF Polyclonal antibody (19548-1-AP)

    IHC staining of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYOF Polyclonal antibody (19548-1-AP)

    IF Staining of HepG2 using 19548-1-AP (same clone as 19548-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Myoferlin antibody (19548-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

MYOF Polyclonal antibody, PBS Only

MYOF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19548-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19548-1-PBS targets MYOF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name myoferlin
Calculated molecular weight 235 kDa
Observed molecular weight 235-250 kDa
GenBank accession numberNM_133337
Gene Symbol MYOF
Gene ID (NCBI) 26509
RRIDAB_10642558
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZM1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR. The antibody can recognize all the 8 isoforms of MYOF.

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