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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYOD1 Polyclonal antibody (18943-1-AP)

    WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MYOD1 Polyclonal antibody (18943-1-AP)

    WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using MYOD1 Polyclonal antibody (18943-1-AP)

    WB analysis of human heart using 18943-1-AP (same clone as 18943-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using MYOD1 Polyclonal antibody (18943-1-AP)

    WB analysis of mouse heart using 18943-1-AP (same clone as 18943-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYOD1 Polyclonal antibody (18943-1-AP)

    WB analysis of mouse skeletal muscle using 18943-1-AP (same clone as 18943-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using MYOD1 Polyclonal antibody (18943-1-AP)

    WB analysis of rat heart using 18943-1-AP (same clone as 18943-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using MYOD1 Polyclonal antibody (18943-1-AP)

    IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using MYOD1 Polyclonal antibody (18943-1-AP)

    IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using MYOD1 Polyclonal antibody (18943-1-AP)

    IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MYOD1 Polyclonal antibody (18943-1-AP)

    IF Staining of HeLa using 18943-1-AP (same clone as 18943-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYOD1 antibody (18943-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18943-1-PBS targets MYOD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYOD1 fusion protein Ag13512 相同性解析による交差性が予測される生物種
Full Name myogenic differentiation 1
Calculated molecular weight 320 aa, 35 kDa
Observed molecular weight 35-45 kDa
GenBank accession numberBC064493
Gene Symbol MYOD1
Gene ID (NCBI) 4654
RRIDAB_10603467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15172
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)