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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MYO1A Polyclonal antibody (17499-1-AP)

    WB analysis of HepG2 using 17499-1-AP

    WB result of MYO1A antibody (17499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1A transfected HepG2 cells.

  • Western Blot (WB) analysis of mouse brain tissue using MYO1A Polyclonal antibody (17499-1-AP)

    WB analysis of mouse brain using 17499-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using MYO1A Polyclonal antibody (17499-1-AP)

    WB analysis of A431 using 17499-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MYO1A Polyclonal antibody (17499-1-AP)

    WB analysis of HepG2 using 17499-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MYO1A Polyclonal antibody (17499-1-AP)

    WB analysis of HeLa using 17499-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MYO1A Polyclonal antibody (17499-1-AP)

    IP experiment of HeLa using 17499-1-AP

    IP result of anti-MYO1A (IP:17499-1-AP, 4ug; Detection:17499-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MYO1A Polyclonal antibody (17499-1-AP)

    IHC staining of human colon cancer using 17499-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17499-1-AP (MYO1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYO1A Polyclonal antibody (17499-1-AP)

    IHC staining of human kidney using 17499-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17499-1-AP (MYO1A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYO1A Polyclonal antibody (17499-1-AP)

    IHC staining of human kidney using 17499-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17499-1-AP (MYO1A antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYO1A Polyclonal antibody (17499-1-AP)

    IF Staining of HepG2 using 17499-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYO1A antibody (17499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MYO1A Polyclonal antibody (17499-1-AP)

    IF Staining of HeLa using 17499-1-AP

    Immunofluorescent analysis of Hela cells, using MYO1A antibody 17499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Featured Product
  • KD/KO Validated

MYO1A Polyclonal antibody

MYO1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17499-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MIHC, DFNA48, Brush border myosin I, BBM-I, BBMI

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, HeLa cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17499-1-AP targets MYO1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11682

Product name: Recombinant human MYO1A protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-350 aa of BC059387

Sequence: MPLLEGSVGVEDLVLLEPLVEESLLKNLQLRYENKEIYTYIGNVVISVNPYQQLPIYGPEFIAKYQDYTFYELKPHIYALANVAYQSLRDRDRDQCILITGESGSGKTEASKLVMSYVAAVCGKGEQVNSVKEQLLQSNPVLEAFGNAKTIRNNNSSRFGKYMDIEFDFKGSPLGGVITNYLLEKSRLVKQLKGERNFHIFYQLLAGADEQLLKALKLERDTTGYAYLNHEVSRVDGMDDASSFRAVQSAMAVIGFSEEEIRQVLEVTSMVLKLGNVLVADEFQASGIPASGIRDGRGVREIGEMVGLNSEEVERALCSRTMETAKEKVVTALNVMQAQYARDALAKNIY

相同性解析による交差性が予測される生物種
Full Name myosin IA
Calculated molecular weight 1043 aa, 118 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC059387
Gene Symbol MYO1A
Gene ID (NCBI) 4640
RRIDAB_2148172
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for MYO1A antibody 17499-1-APDownload protocol
IHC protocol for MYO1A antibody 17499-1-APDownload protocol
IP protocol for MYO1A antibody 17499-1-APDownload protocol
WB protocol for MYO1A antibody 17499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Rep Med

Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer

Authors - Yi Zhao
View Article
mouseWB

Clin Sci (Lond)

A new mechanism of arterial calcification in diabetes: interaction between H3K18 lactylation and CHI3L1

Authors - Yi Zhu
View Article
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