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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MYO19 Polyclonal antibody (23906-1-AP)

    WB analysis of HeLa using 23906-1-AP (same clone as 23906-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23906-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MYO19 Polyclonal antibody (23906-1-AP)

    WB analysis of A549 using 23906-1-AP (same clone as 23906-1-PBS)

    WB result of MYO19 antibody (23906-1-AP, 1:300) with si-Control and si-MYO19 transfected A549 cells. This data was developed using the same antibody clone with 23906-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COS-7 cells using MYO19 Polyclonal antibody (23906-1-AP)

    WB analysis of COS-7 using 23906-1-AP (same clone as 23906-1-PBS)

    COS-7 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23906-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MYO19 Polyclonal antibody (23906-1-AP)

    WB analysis of A549 using 23906-1-AP (same clone as 23906-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYO19 Polyclonal antibody (23906-1-AP)

    IHC staining of human kidney using 23906-1-AP (same clone as 23906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MYO19 Polyclonal antibody (23906-1-AP)

    IHC staining of rat kidney using 23906-1-AP (same clone as 23906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using MYO19 Polyclonal antibody (23906-1-AP)

    IHC staining of rat testis using 23906-1-AP (same clone as 23906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23906-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MYO19 Polyclonal antibody (23906-1-AP)

    IF Staining of A549 using 23906-1-AP (same clone as 23906-1-PBS)

    Immunofluorescent analysis of A549 cells using 23906-1-AP (MYO19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23906-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MYO19 Polyclonal antibody, PBS Only

MYO19 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23906-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23906-1-PBS targets MYO19 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, monkey samples.

Tested Reactivity human, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20995

Product name: Recombinant human MYO19 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 441-770 aa of BC008900

Sequence: EACGLVETIHISAAGFPIRVSHRNFVERYKLLRRLHPCTSSGPDSPYPAKGLPEWCPHSEEATLEPLIQDILHTLPVLTQAAAITGDSAEAMPAPMHCGRTKVFMTDSMLELLECGRARVLEQCARCIQGGWRRHRHREQERQWRAVMLIQAAIRSWLTRKHIQRLHAAATVIKRAWQKWRIRMACLAAKELDGVEEKHFSQAPCSLSTSPLQTRLLEAIIRLWPLGLVLANTAMGVGSFQRKLVVWACLQLPRGSPSSYTVQTAQDQAGVTSIRALPQGSIKFHCRKSPLRYADICPEPSPYSITGFNQILLERHRLIHVTSSAFTGLG

相同性解析による交差性が予測される生物種
Full Name myosin XIX
Calculated molecular weight 970 aa, 109 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC008900
Gene Symbol MYO19
Gene ID (NCBI) 80179
RRIDAB_2879357
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96H55
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYO19 is also named as MYOHD1. It is Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992) and required for mitochondrial inheritance during mitosis (PubMed:25447992). MYO19 can enable actin binding activity and be involved in regulation of cytokinesis and regulation of mitochondrial fission. MYO19 can be detected as about 109 kDa.

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