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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYO18A Monoclonal antibody (68868-1-Ig)

    WB analysis using 68868-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68868-1-Ig (MYO18A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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MYO18A Monoclonal antibody

MYO18A Monoclonal Antibody for WB, ELISA
Cat No. 68868-1-Ig
Clone No.1A2B12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

myosinXVIIIA, myosin XVIIIA, Molecule associated with JAK3 N-terminus, KIAA0216, CD245

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHaCaT cells, A431 cells, SW 1990 cells, HT-29 cells, MDA-MB-468 cells, HeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68868-1-Ig targets MYO18A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYO18A fusion protein Ag6185 相同性解析による交差性が予測される生物種
Full Name myosin XVIIIA
Calculated molecular weight 233 kDa
Observed molecular weight 230 kDa, 190 kDa
GenBank accession numberBC039612
Gene Symbol MYO18A
Gene ID (NCBI) 399687
RRIDAB_3670442
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92614
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for MYO18A antibody 68868-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols