Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Jurkat using 14611-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14611-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14611-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 14611-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 14611-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14611-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14611-1-AP
IP result of anti-MYO18A (IP:14611-1-AP, 3ug; Detection:14611-1-AP 1:300) with HeLa cells lysate 3000ug.
IHC staining of mouse skeletal muscle using 14611-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 14611-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 14611-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

MYO18A Polyclonal antibody

MYO18A Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14611-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

CD245, KIAA0216, Molecule associated with JAK3 N-terminus, myosin XVIIIA, myosinXVIIIA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse heart tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 14 publications below
IHC	See 1 publications below
IF	See 5 publications below

Product Information

14611-1-AP targets MYO18A in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6185 Product name: Recombinant human MYO18A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1601-2002 aa of BC039612 Sequence: TLSDQVNRRDFESEKRLRKDLKRTKALLADAQLMLDHLKNSAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQAQLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFERTQVKRLESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYLSDSDTEAKLTETNA 相同性解析による交差性が予測される生物種
Full Name	myosin XVIIIA
Calculated molecular weight	233 kDa
Observed molecular weight	230 kDa, 190 kDa
GenBank accession number	BC039612
Gene Symbol	MYO18A
Gene ID (NCBI)	399687
RRID	AB_2201447
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92614
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.

Protocols

Product Specific Protocols
IHC protocol for MYO18A antibody 14611-1-AP	Download protocol
IP protocol for MYO18A antibody 14611-1-AP	Download protocol
WB protocol for MYO18A antibody 14611-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. Authors - Xiaochao Tan KD Validated View Article
human	WB,IHC	Cell Death Differ The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma. Authors - Jialiang Liu KD Validated View Article
mouse	FC (Intra)	Thorax Advanced MicroRNA delivery for lung inflammatory therapy: surfactant protein A controls cellular internalisation and degradation of extracellular vesicles Authors - Miji Kim View Article
human	WB,IF	Cell Syst A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection. Authors - Pierre M Jean Beltran KD Validated View Article
human	WB	Oncoimmunology Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes. Authors - A De Masson View Article
human	WB	Diabetes Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding. Authors - Duc T Tran View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 14611-1-AP

WB analysis of K-562 using 14611-1-AP

WB analysis of HeLa using 14611-1-AP

WB analysis of NIH/3T3 using 14611-1-AP

WB analysis of Neuro-2a using 14611-1-AP

WB analysis of HeLa using 14611-1-AP

IP experiment of HeLa using 14611-1-AP

IHC staining of mouse skeletal muscle using 14611-1-AP

IHC staining of mouse heart using 14611-1-AP

IHC staining of mouse heart using 14611-1-AP

MYO18A Polyclonal antibody

MYO18A Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.

The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.

Advanced MicroRNA delivery for lung inflammatory therapy: surfactant protein A controls cellular internalisation and degradation of extracellular vesicles

A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection.

Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes.

Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding.