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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYNN Polyclonal antibody (13369-1-AP)

    WB analysis using 13369-1-AP (same clone as 13369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13369-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using MYNN Polyclonal antibody (13369-1-AP)

    WB analysis of mouse brain using 13369-1-AP (same clone as 13369-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYNN Polyclonal antibody (13369-1-AP)

    IHC staining of human placenta using 13369-1-AP (same clone as 13369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYNN Polyclonal antibody (13369-1-AP)

    IHC staining of human placenta using 13369-1-AP (same clone as 13369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13369-1-PBS in a different storage buffer formulation.

MYNN Polyclonal antibody, PBS Only

MYNN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13369-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13369-1-PBS targets MYNN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4188

Product name: Recombinant human MYNN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 303-610 aa of BC033620

Sequence: MCNTCGKVFSEASSLRRHMRIHKGVKPYVCHLCGKAFTQCNQLKTHVRTHTGEKPYKCELCDKGFAQKCQLVFHSRMHHGEEKPYKCDVCNLQFATSSNLKIHARKHSGEKPYVCDRCGQRFAQASTLTYHVRRHTGEKPYVCDTCGKAFAVSSSLITHSRKHTGEKPYICGICGKSFISSGELNKHFRSHTGERPFICELCGNSYTDIKNLKKHKTKVHSGADKTLDSSAEDHTLSEQDSIQKSPLSETMDVKPSDMTLPLALPLGTEDHHMLLPVTDTQSPTSDTLLRSTVNGYSEPQLIFLQQLY

相同性解析による交差性が予測される生物種
Full Name myoneurin
Calculated molecular weight 610 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC033620
Gene Symbol MYNN
Gene ID (NCBI) 55892
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPC7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Myoneurin (MYNN) gene locates on 3q26.2 and encodes a member of the BTB/POZ and zinc finger (ZF) domain-containing protein family that is involved in the control of gene expression. MYNN is widely expressed in various tissue types in mammals. MYNN has 4 isoforms with the molecular mass of 10, 57, 65-69 kDa. (PMID: 30120764)

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