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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYNN Polyclonal antibody (13369-1-AP)

    WB analysis using 13369-1-AP (same clone as 13369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13369-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using MYNN Polyclonal antibody (13369-1-AP)

    WB analysis of mouse brain using 13369-1-AP (same clone as 13369-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYNN Polyclonal antibody (13369-1-AP)

    IHC staining of human placenta using 13369-1-AP (same clone as 13369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYNN Polyclonal antibody (13369-1-AP)

    IHC staining of human placenta using 13369-1-AP (same clone as 13369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13369-1-PBS in a different storage buffer formulation.

MYNN Polyclonal antibody, PBS Only

MYNN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13369-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13369-1-PBS targets MYNN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4188

Product name: Recombinant human MYNN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 303-610 aa of BC033620

Sequence: MCNTCGKVFSEASSLRRHMRIHKGVKPYVCHLCGKAFTQCNQLKTHVRTHTGEKPYKCELCDKGFAQKCQLVFHSRMHHGEEKPYKCDVCNLQFATSSNLKIHARKHSGEKPYVCDRCGQRFAQASTLTYHVRRHTGEKPYVCDTCGKAFAVSSSLITHSRKHTGEKPYICGICGKSFISSGELNKHFRSHTGERPFICELCGNSYTDIKNLKKHKTKVHSGADKTLDSSAEDHTLSEQDSIQKSPLSETMDVKPSDMTLPLALPLGTEDHHMLLPVTDTQSPTSDTLLRSTVNGYSEPQLIFLQQLY

相同性解析による交差性が予測される生物種
Full Name myoneurin
Calculated molecular weight 610 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC033620
Gene Symbol MYNN
Gene ID (NCBI) 55892
RRIDAB_2923559
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPC7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Myoneurin (MYNN) gene locates on 3q26.2 and encodes a member of the BTB/POZ and zinc finger (ZF) domain-containing protein family that is involved in the control of gene expression. MYNN is widely expressed in various tissue types in mammals. MYNN has 4 isoforms with the molecular mass of 10, 57, 65-69 kDa. (PMID: 30120764)

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