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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using MYLK3 Recombinant antibody (83673-3-RR)

    WB analysis of L02 using 83673-3-RR (same clone as 83673-3-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 83673-3-RR (MYLK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83673-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using MYLK3 Recombinant antibody (83673-3-RR)

    WB analysis of human heart using 83673-3-RR (same clone as 83673-3-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 83673-3-RR (MYLK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83673-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83673-3-RR

    Affinity and Kinetic Characterization of 83673-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83673-3-RR against Human MYLK3 were performed. The affinity constant is below 1 pM.

MYLK3 Recombinant monoclonal antibody, PBS Only

MYLK3 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83673-3-PBS
Clone No.240762G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MLCK, EC:2.7.11.18, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, caMLCK

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83673-3-PBS targets MYLK3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16137

Product name: Recombinant human MYLK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC109097

Sequence: MDTKLNMLNEKVDQLLHFQEDVTEKLQSMCRDMGHLERGLHRLEASRAPGPGGADGVPHIDTQAGWPEVLELVRAMQQDAAQHGARLEALFRMVAAVDRAIALVGATFQKSKVADFLMQGRVPWRRGSPGDSPEENKERVEEEGGKPKHVLSTSGLQSDAREPGEESQKADVLEGTAERLPPIRASGLGADPAQAVVSPGQGDGVPGPAQAFPGHLPLPTKVEAKAPETPSENLRTGLELAPAPGRVNVVSPSLEVAPGAGQGASSSRPDPEPLEEGTRLTPGPGPQCPGPPGLPAQARATHSGGETPPRISIHIQEMDTPGEMLMTGRGSLGPTLTTEAPAAAQPGKQG

相同性解析による交差性が予測される生物種
Full Name myosin light chain kinase 3
Calculated molecular weight 819 aa, 88 kDa
GenBank accession numberBC109097
Gene Symbol MYLK3
Gene ID (NCBI) 91807
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ32MK0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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