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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis of human skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis of Jurkat using 21173-1-AP (same clone as 21173-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

MYLK2 Polyclonal antibody, PBS Only

MYLK2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21173-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21173-1-PBS targets MYLK2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15424

Product name: Recombinant human MYLK2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-300 aa of BC069627

Sequence: MATENGAVELGIQNPSTDKAPKGPTGERPLAAGKDPGPPDPKKAPDPPTLKKDAKAPASEKGDGTLAQPSTSSQGPKGEGDRGGGPAEGSAGPPAALPQQTATPETSVKKPKAEQGASGSQDPGKPRVGKKAAEGQAAARRGSPAFLHSPSCPAIISSSEKLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVC

相同性解析による交差性が予測される生物種
Full Name myosin light chain kinase 2
Calculated molecular weight 596 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC069627
Gene Symbol MYLK2
Gene ID (NCBI) 85366
RRIDAB_10733123
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1R3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYLK2, also named as MLCK2, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. MYLK2 catalyze the reaction: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. MYLK2 is implicated in the level of global muscle contraction and cardiac function. MYLK2 phosphorylates a specific serine in the N-terminus of a myosin light chain. Mutation of MYLK2 will cause the cardiomyopathy familial hypertrophic (CMH).

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