Validation Data Gallery
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Tested Applications
| Positive WB detected in | human cerebellum tissue, CHO cells |
| Positive IHC detected in | human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
15455-1-AP targets MYLIP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.
| Tested Reactivity | human, mouse, rat, hamster |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7720 Product name: Recombinant human MYLIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC002860 Sequence: MLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGLQFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHIKEALLAGHLLCSPEQAVELSALLAQTKFGDYNQNTAKYNYEELCAKELSSATLNSIVAKHKELEGTSQASAEYQVLQIVSAMENYGIEWHSVRDSEGQKLLIGVGPEGISICKDDFSPINRIAYPVVQMATQSGKNVYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRALYNAGVVDLVSRNNQSPSHSPLK 相同性解析による交差性が予測される生物種 |
| Full Name | myosin regulatory light chain interacting protein |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 46-50 kDa |
| GenBank accession number | BC002860 |
| Gene Symbol | MYLIP |
| Gene ID (NCBI) | 29116 |
| RRID | AB_2235610 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WY64 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYLIP antibody 15455-1-AP | Download protocol |
| WB protocol for MYLIP antibody 15455-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Lett Appl Microbiol Xinnaokang improves cecal microbiota and lipid metabolism to target atherosclerosis. | ||