Validation Data Gallery

Filter: AllWBIHC

WB analysis of human cerebellum using 15455-1-AP
human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15455-1-AP (MYLIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of CHO using 15455-1-AP
CHO cells were subjected to SDS PAGE followed by western blot with 15455-1-AP (MYLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 15455-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

MYLIP Polyclonal antibody

MYLIP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15455-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, ELISA

Synonyms

BZF1, Idol, MIR, MYLIP, PP5242

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human cerebellum tissue, CHO cells
Positive IHC detected in	human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

15455-1-AP targets MYLIP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity	human, mouse, rat, hamster
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MYLIP fusion protein Ag7720 相同性解析による交差性が予測される生物種
Full Name	myosin regulatory light chain interacting protein
Calculated molecular weight	50 kDa
Observed molecular weight	46-50 kDa
GenBank accession number	BC002860
Gene Symbol	MYLIP
Gene ID (NCBI)	29116
RRID	AB_2235610
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WY64
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MYLIP antibody 15455-1-AP	Download protocol
IHC protocol for MYLIP antibody 15455-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Elife SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Authors - Chen Xiao-Wei XW View Article
mouse	WB	Lett Appl Microbiol Xinnaokang improves cecal microbiota and lipid metabolism to target atherosclerosis. Authors - R Yang View Article
human	WB	Heliyon Identification of ubiquitin markers for survival and prognosis of ovarian cancer Authors - Yiwen Feng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human cerebellum using 15455-1-AP

WB analysis of CHO using 15455-1-AP

IHC staining of human testis using 15455-1-AP

IHC staining of human testis using 15455-1-AP

IHC staining of human skin using 15455-1-AP

IHC staining of human skin using 15455-1-AP

IHC staining of human brain using 15455-1-AP

IHC staining of human brain using 15455-1-AP

IHC staining of human liver using 15455-1-AP

IHC staining of human liver using 15455-1-AP

IHC staining of human spleen using 15455-1-AP

IHC staining of human spleen using 15455-1-AP

IHC staining of human lung using 15455-1-AP

IHC staining of human lung using 15455-1-AP

IHC staining of human ovary using 15455-1-AP

IHC staining of human ovary using 15455-1-AP

IHC staining of human heart using 15455-1-AP