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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYL6 Polyclonal antibody (18158-1-AP)

    WB analysis using 18158-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18158-1-AP (MYL6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYL6 Polyclonal antibody (18158-1-AP)

    IHC staining of mouse heart using 18158-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18158-1-AP (MYL6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MYL6 Polyclonal antibody (18158-1-AP)

    IF Staining of HeLa using 18158-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (18158-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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MYL6 Polyclonal antibody

MYL6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18158-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MYL 6, MLC-3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse uterus tissue, rat uterus tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18158-1-AP targets MYL6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL6 fusion protein Ag12964 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 6, alkali, smooth muscle and non-muscle
Calculated molecular weight 17 kDa
Observed molecular weight15-20 kDa
GenBank accession numberBC006781
Gene Symbol MYL6
Gene ID (NCBI) 4637
RRIDAB_3085557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60660
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).

Protocols

Product Specific Protocols
WB protocol for MYL6 antibody 18158-1-APDownload protocol
IHC protocol for MYL6 antibody 18158-1-APDownload protocol
IF protocol for MYL6 antibody 18158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols