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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

MYL5 Polyclonal antibody

MYL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14249-1-AP

Synonyms

MYL5, MyLC 2, MYLC2, Myosin light chain 5

Validation Data Gallery

Applications:
  • WB analysis using 14249-1-AP

    WB analysis using 14249-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14249-1-AP (MYL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 14249-1-AP

    WB analysis of human brain using 14249-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14249-1-AP (MYL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 14249-1-AP

    IHC staining of human cervical cancer using 14249-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL5 antibody included
  • IHC staining of human cervical cancer using 14249-1-AP

    IHC staining of human cervical cancer using 14249-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL5 antibody included
  • IHC staining of mouse kidney using 14249-1-AP

    IHC staining of mouse kidney using 14249-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL5 antibody included
  • IHC staining of mouse kidney using 14249-1-AP

    IHC staining of mouse kidney using 14249-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL5 antibody included
  • IHC staining of human cervical cancer using 14249-1-AP

    IHC staining of human cervical cancer using 14249-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL5 antibody included
  • IHC staining of human cervical cancer using 14249-1-AP

    IHC staining of human cervical cancer using 14249-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14249-1-AP (MYL5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL5 antibody included

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human brain tissue, rat skeletal muscle tissue
Positive IHC detected inhuman cervical cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYL5 Ready-To-Use IHC Kit. MYL5 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14249-1-AP targets MYL5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL5 fusion protein Ag5522 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 5, regulatory
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC040050
Gene symbol MYL5
Gene ID (NCBI) 4636
RRIDAB_2266762
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYL5 antibody 14249-1-APDownload protocol
IHC protocol for MYL5 antibody 14249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Cytoskeleton (Hoboken)

The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division.

Authors - Ivan Ramirez
  • KD Validated
View Article