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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYL12A Polyclonal antibody

MYL12A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16287-1-AP

Synonyms

MLC 2B, MLCB, MRLC3, MYL12A, MYL2B, Myosin RLC, RLC

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 16287-1-AP

    WB analysis of NIH/3T3 using 16287-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16287-1-AP

    WB analysis of HeLa using 16287-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human skeletal muscle using 16287-1-AP

    WB analysis of human skeletal muscle using 16287-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse bladder using 16287-1-AP

    WB analysis of mouse bladder using 16287-1-AP

    mouse bladder tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 16287-1-AP

    WB analysis of NIH/3T3 using 16287-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 16287-1-AP

    WB analysis of Jurkat using 16287-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16287-1-AP

    WB analysis of HEK-293 using 16287-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 16287-1-AP

    WB analysis of mouse heart using 16287-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 16287-1-AP

    IHC staining of human heart using 16287-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MYL12A antibody included
  • IHC staining of human heart using 16287-1-AP

    IHC staining of human heart using 16287-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MYL12A antibody included
  • IHC staining of human skeletal muscle using 16287-1-AP

    IHC staining of human skeletal muscle using 16287-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MYL12A antibody included
  • IHC staining of human skeletal muscle using 16287-1-AP

    IHC staining of human skeletal muscle using 16287-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MYL12A antibody included

Tested Applications

Positive WB detected inNIH/3T3 cells, human skeletal muscle tissue, mouse bladder tissue, Jurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYL12A Ready-To-Use IHC Kit. MYL12A primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16287-1-AP targets MYL12A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL12A fusion protein Ag9413 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 12A, regulatory, non-sarcomeric
Calculated molecular weight 171 aa, 20 kDa
Observed molecular weight 19-20 kDa
GenBank accession numberBC016372
Gene symbol MYL12A
Gene ID (NCBI) 10627
RRIDAB_2878238
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYL12A antibody 16287-1-APDownload protocol
IHC protocol for MYL12A antibody 16287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Biochem J

Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli.

Authors - Shcherbakova Olga V OV
View Article
mouseWB

Front Physiol

Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction.

Authors - Jie Sun
View Article
humanIHC

Biofizika

[Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium].

Authors - Marchenko A V AV
View Article