Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
15396-1-AP targets MYL10 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7594 Product name: Recombinant human MYL10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC002778 Sequence: MLLRLVSNSWPQVILPPRPPKVLGLQAPRRARKRAEGTASSNVFSMFDQSQIQEFKESLALSPRLERNGMISAHCNLCLTGSSNSPASASQAFTIMDQNRDGFIDKEDLRDTFAALGRINVKNEELEAMVKEAPGPINFTVFLTMFGEKLKGTDPEETILHAFKVFDTEGKGFVKADVIKEKLMTQADRFSEEEVKQMFAAFPPDVCGNLDYRNLCYVITHGEEKD 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, light chain 10, regulatory |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC002778 |
| Gene Symbol | MYL10 |
| Gene ID (NCBI) | 93408 |
| RRID | AB_3741865 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BUA6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
In non-muscle cells, MLCs are either regulatory (RLC) or essential ELCs. The human RLC gene family encompasses MYL2, MYL5, MYL9, MYL10, MYL11, MYL12A, and MYL12B , with the ELC gene family including MYL1 , MYL3, MYL4, and MYL6(PMID: 39768172). MYL10, also known as PLRLC, encodes for an RLC that might be a lymphocyte specific precursor. MYL10 is a regulatory myosin light chain that is expressed in pre-B cells from adult bone marrow, but not those from fetal liver, upon exposure to IL-7(PMID: 1628631).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL10 antibody 15396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |