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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYH9 Recombinant antibody (81204-1-RR)

    WB analysis using 81204-1-RR (same clone as 81204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81204-1-RR (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MYH9 Recombinant antibody (81204-1-RR)

    IHC staining of human colon cancer using 81204-1-RR (same clone as 81204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MYH9 Recombinant antibody (81204-1-RR)

    IHC staining of rat kidney using 81204-1-RR (same clone as 81204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 81204-1-RR (MYH9 antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYH9 Recombinant antibody (81204-1-RR)

    IF Staining of HepG2 using 81204-1-RR (same clone as 81204-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (81204-1-RR, Clone: 3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81204-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81204-1-RR

    Affinity and Kinetic Characterization of 81204-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81204-1-RR against Human MYH9 were performed. The affinity constant is 0.297 nM.

MYH9 Recombinant monoclonal antibody, PBS Only

MYH9 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81204-1-PBS
Clone No.3E1

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81204-1-PBS targets MYH9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1612

Product name: Recombinant human MYH9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1741-1960 aa of BC011915

Sequence: EQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE

相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 9, non-muscle
Calculated molecular weight 227 kDa
Observed molecular weight224-227 kDa
GenBank accession numberBC011915
Gene Symbol MYH9
Gene ID (NCBI) 4627
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35579
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

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