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Validation Data Gallery

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  • Western Blot (WB) analysis of rat skeletal muscle tissue using MYH8-Specific Recombinant antibody (84594-1-RR)

    WB analysis of rat skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84594-1-RR (MYH8-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using MYH8-Specific Recombinant antibody (84594-1-RR)

    WB analysis of human heart using 84594-1-RR (same clone as 84594-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 84594-1-RR (MYH8-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYH8-Specific Recombinant antibody (84594-1-RR)

    IHC staining of mouse skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84594-1-RR (MYH8-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYH8-Specific Recombinant antibody (84594-1-RR)

    IHC staining of mouse skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84594-1-RR (MYH8-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYH8-Specific Recombinant antibody (84594-1-RR)

    IF Staining of mouse skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH8-Specific antibody (84594-1-RR, Clone: 241539A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYH8-Specific Recombinant antibody (84594-1-RR)

    IF Staining of mouse skeletal muscle using 84594-1-RR (same clone as 84594-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH8-Specific antibody (84594-1-RR, Clone: 241539A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84594-1-PBS in a different storage buffer formulation.

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MYH8-Specific Recombinant antibody, PBS Only

MYH8-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84594-1-PBS
Clone No.241539A1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

MYH8, 241539A1, Myosin-8, Myosin heavy chain 8, Myosin 8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84594-1-PBS targets MYH8-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 8, skeletal muscle, perinatal
Calculated molecular weight 223 kDa
Observed molecular weight220-230 kDa
GenBank accession numberNM_002472
Gene Symbol MYH8
Gene ID (NCBI) 4626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13535
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.