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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYH8-Specific Polyclonal antibody (18988-1-AP)

    WB analysis using 18988-1-AP

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 18988-1-AP (MYH8-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYH8-Specific Polyclonal antibody (18988-1-AP)

    IHC staining of mouse skeletal muscle using 18988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18988-1-AP (MYH8-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MYH8-Specific Polyclonal antibody

MYH8-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18988-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

MYH8, MYH8-Specific, MyHC peri, MyHC perinatal, MyHC pn, Myosin 8, Myosin heavy chain 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18988-1-AP targets MYH8-Specific in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 8, skeletal muscle, perinatal
Calculated molecular weight 223 kDa
Observed molecular weight220-230 kDa
GenBank accession numberNM_002472
Gene Symbol MYH8
Gene ID (NCBI) 4626
RRIDAB_3085572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13535
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH8 plays a role in muscle contraction. It is required for cytoskeleton organization. The antibody is specific to MYH8 and MYH2.

Protocols

Product Specific Protocols
WB protocol for MYH8-Specific antibody 18988-1-APDownload protocol
IHC protocol for MYH8-Specific antibody 18988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols