Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 31 publications below |
| IHC | See 2 publications below |
| IF | See 10 publications below |
Product Information
20140-1-AP targets MYH4-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, heavy chain 4, skeletal muscle |
| Calculated molecular weight | 223 kDa |
| Observed molecular weight | 230 kDa |
| GenBank accession number | NM_017533 |
| Gene Symbol | MYH4 |
| Gene ID (NCBI) | 4622 |
| RRID | AB_10666434 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y623 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myosin is one of the most important skeletal muscle proteins. MYH4 functions muscle contraction. The antibody is specific to MYH4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| IHC protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| IF protocol for MYH4-Specific antibody 20140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction | ||
Nat Commun The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization. | ||
Development The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition | ||
Am J Pathol Klotho null mutation involvement in adenosine A2B receptor-related skeletal muscle degeneration | ||
Development The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition. | ||
Int J Mol Sci Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle |