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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYH13 Polyclonal antibody

MYH13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22286-1-AP

Synonyms

MYH13, MyHC eo, Myosin 13, Myosin heavy chain 13

Validation Data Gallery

Applications:
  • WB analysis using 22286-1-AP

    WB analysis using 22286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22286-1-AP (MYH13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 22286-1-AP

    IHC staining of mouse skeletal muscle using 22286-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22286-1-AP (MYH13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH13 antibody included

Tested Applications

Positive WB detected inmouse eye tissue, rat eye tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYH13 Ready-To-Use IHC Kit. MYH13 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

22286-1-AP targets MYH13 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 13, skeletal muscle
Calculated molecular weight 224 kDa
Observed molecular weight230-240 kDa
GenBank accession numberNM_003802
Gene symbol MYH13
Gene ID (NCBI) 8735
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYH13 antibody 22286-1-APDownload protocol
IHC protocol for MYH13 antibody 22286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols