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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using MYCN Polyclonal antibody (10159-2-AP)

    WB analysis of human placenta using 10159-2-AP (same clone as 10159-2-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using MYCN Polyclonal antibody (10159-2-AP)

    WB analysis of human placenta using 10159-2-AP (same clone as 10159-2-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using MYCN Polyclonal antibody (10159-2-AP)

    WB analysis of C6 using 10159-2-AP (same clone as 10159-2-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using MYCN Polyclonal antibody (10159-2-AP)

    WB analysis of SKOV-3 using 10159-2-AP (same clone as 10159-2-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using MYCN Polyclonal antibody (10159-2-AP)

    WB analysis of SH-SY5Y using 10159-2-AP (same clone as 10159-2-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using MYCN Polyclonal antibody (10159-2-AP)

    WB analysis of mouse brain using 10159-2-AP (same clone as 10159-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using MYCN Polyclonal antibody (10159-2-AP)

    WB analysis of rat brain using 10159-2-AP (same clone as 10159-2-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using MYCN Polyclonal antibody (10159-2-AP)

    IHC staining of human thyroid cancer using 10159-2-AP (same clone as 10159-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP (MYCN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using MYCN Polyclonal antibody (10159-2-AP)

    IHC staining of human thyroid cancer using 10159-2-AP (same clone as 10159-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP (MYCN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MYCN Polyclonal antibody, PBS Only

MYCN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10159-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10159-2-PBS targets MYCN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0193

Product name: Recombinant human MYCN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 289-464 aa of BC002712

Sequence: SNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC

相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Calculated molecular weight 50 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC002712
Gene Symbol MYCN
Gene ID (NCBI) 4613
RRIDAB_2266881
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04198
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )

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