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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MYCL1 Recombinant antibody (82881-1-RR)

    IF Staining of U2OS using 82881-1-RR (same clone as 82881-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCL1 antibody (82881-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82881-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MYCL1 Recombinant antibody (82881-1-RR)

    FC experiment of HeLa using 82881-1-RR (same clone as 82881-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MYCL1 Recombinant antibody (82881-1-RR, Clone:230104D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82881-1-PBS in a different storage buffer formulation.

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MYCL1 Recombinant antibody, PBS Only

MYCL1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82881-1-PBS
Clone No.230104D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Protein L-Myc, MYCL, LMYC, Class E basic helix-loop-helix protein 38, bHLHe38

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82881-1-PBS targets MYCL1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYCL1 fusion protein Ag30335 相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian)
Calculated molecular weight 40 kDa
GenBank accession numberBC011864
Gene Symbol MYCL1
Gene ID (NCBI) 4610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12524
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.