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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of human brain using 27951-1-AP (same clone as 27951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of human brain using 27951-1-AP (same clone as 27951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of rat brain using 27951-1-AP (same clone as 27951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of rat brain using 27951-1-AP (same clone as 27951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of mouse brain using 27951-1-AP (same clone as 27951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of mouse brain using 27951-1-AP (same clone as 27951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MYCBP2 Polyclonal antibody (27951-1-AP)

    IF Staining of U2OS using 27951-1-AP (same clone as 27951-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCBP2 antibody (27951-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using MYCBP2 Polyclonal antibody (27951-1-AP)

    FC experiment of U2OS using 27951-1-AP (same clone as 27951-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug MYCBP2 Polyclonal antibody (27951-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 27951-1-PBS in a different storage buffer formulation.

MYCBP2 Polyclonal antibody, PBS Only

MYCBP2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 27951-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27951-1-PBS targets MYCBP2 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27228

Product name: Recombinant human MYCBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2672-2880 aa of NM_015057

Sequence: MGNSKGDRGNISTSSKPASTSGKSELSSKHSRSLKPDGRMSRTTADQKKPRGTESLSASESLILKSDAAKLRSDSHSRSLSPNHNTLQTLKSDGRMPSSSRAESPGPGSRLSSPKPKTLPANRSSPSGASSPRSSSPHDKNLPQKSTAPVKTKLDPPRERSKSDSYTLDPDTLRKKKMPLTEPLRGRSTSPKPKSVPKDSTDSPGSENRA

相同性解析による交差性が予測される生物種
Full Name MYC binding protein 2
Calculated molecular weight 510 kDa
GenBank accession numberNM_015057
Gene Symbol MYCBP2
Gene ID (NCBI) 23077
RRIDAB_2881020
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75592
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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