Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IF | See 1 publications below |
| IP | See 5 publications below |
Product Information
60003-1-Ig targets MYC-tag in WB, IF, IP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Myc tag |
| Gene Symbol | Myc tag |
| Gene ID (NCBI) | 99 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | N/A |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Metabolic regulation of telomere silencing by SESAME complex-catalyzed H3T11 phosphorylation. | ||
Cell Death Differ Bcl-3 promotes TNF-induced hepatocyte apoptosis by regulating the deubiquitination of RIP1 | ||
Autophagy Chaperone-mediated autophagy controls the turnover of E3 ubiquitin ligase MARCHF5 and regulates mitochondrial dynamics. | ||
Plant Cell Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering. | ||
Proc Natl Acad Sci U S A Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP. | ||