Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein protein, Recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16286-1-AP targets MYC tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, pig, monkey, recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9409 Product name: Recombinant MYC-tag protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: EQKLISEEDLEQKLISEEDLEQKLISEEDL 相同性解析による交差性が予測される生物種 |
| Full Name | MYC tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11182162 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | MYCTAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.
What is the molecular weight of myc?
1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).
What are the applications for myc tag?
The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106).
What is the structure of myc tag?
The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYC tag antibody 16286-1-AP | Download protocol |
| IP protocol for MYC tag antibody 16286-1-AP | Download protocol |
| WB protocol for MYC tag antibody 16286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Signal Transduct Target Ther The E3 ligase RNF5 restricts SARS-CoV-2 replication by targeting its envelope protein for degradation | ||
Signal Transduct Target Ther Glucosidase alpha neutral C promotes influenza virus replication by inhibiting proteosome-dependent degradation of hemagglutinin | ||
Signal Transduct Target Ther BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability. | ||
Signal Transduct Target Ther Family with sequence similarity 114 member A1 orchestrates immune evasion in triple-negative breast cancer | ||
Signal Transduct Target Ther The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. |