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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYBPC3 Monoclonal antibody, PBS Only

MYBPC3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C8F8

Cat no : 67608-1-PBS

Synonyms

Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 67608-1-Ig (same clone as 67608-1-PBS)

    WB analysis of mouse heart using 67608-1-Ig (same clone as 67608-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • WB analysis of H9C2 using 67608-1-Ig (same clone as 67608-1-PBS)

    WB analysis of H9C2 using 67608-1-Ig (same clone as 67608-1-PBS)

    H9C2 cells were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit heart using 67608-1-Ig (same clone as 67608-1-PBS)

    WB analysis of rabbit heart using 67608-1-Ig (same clone as 67608-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • WB analysis of pig heart using 67608-1-Ig (same clone as 67608-1-PBS)

    WB analysis of pig heart using 67608-1-Ig (same clone as 67608-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67608-1-Ig (same clone as 67608-1-PBS)

    IHC staining of human heart using 67608-1-Ig (same clone as 67608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 67608-1-Ig (same clone as 67608-1-PBS)

    IHC staining of rat heart using 67608-1-Ig (same clone as 67608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • IF Staining of rat heart using 67608-1-Ig (same clone as 67608-1-PBS)

    IF Staining of rat heart using 67608-1-Ig (same clone as 67608-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using MYBPC3 antibody (67608-1-Ig, Clone: 3C8F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

67608-1-PBS targets MYBPC3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYBPC3 fusion protein Ag15848 相同性解析による交差性が予測される生物種
Full Name myosin binding protein C, cardiac
Calculated molecular weight 1274 aa, 141 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC151211
Gene symbol MYBPC3
Gene ID (NCBI) 4607
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).