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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using MYBPC3 Monoclonal antibody (67608-1-Ig)

    WB analysis of mouse heart using 67608-1-Ig (same clone as 67608-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of H9C2 cells using MYBPC3 Monoclonal antibody (67608-1-Ig)

    WB analysis of H9C2 using 67608-1-Ig (same clone as 67608-1-PBS)

    H9C2 cells were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit heart tissue using MYBPC3 Monoclonal antibody (67608-1-Ig)

    WB analysis of rabbit heart using 67608-1-Ig (same clone as 67608-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using MYBPC3 Monoclonal antibody (67608-1-Ig)

    WB analysis of pig heart using 67608-1-Ig (same clone as 67608-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MYBPC3 Monoclonal antibody (67608-1-Ig)

    IHC staining of human heart using 67608-1-Ig (same clone as 67608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using MYBPC3 Monoclonal antibody (67608-1-Ig)

    IHC staining of rat heart using 67608-1-Ig (same clone as 67608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using MYBPC3 Monoclonal antibody (67608-1-Ig)

    IF Staining of rat heart using 67608-1-Ig (same clone as 67608-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using MYBPC3 antibody (67608-1-Ig, Clone: 3C8F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67608-1-PBS in a different storage buffer formulation.

MYBPC3 Monoclonal antibody, PBS Only

MYBPC3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67608-1-PBS
Clone No.3C8F8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

3C8F8, Cardiac MyBP C, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67608-1-PBS targets MYBPC3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag15848

Product name: Recombinant human MYBPC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-328 aa of BC151211

Sequence: MPEPGKKPVSAFSKKPRSVEVAAGSPAVFEAETERAGVKVRWQRGGSDISASNKYGLATEGTRHTLTVREVGPADQGSYAVIAGSSKVKFDLKVIEAEKAEPMLAPAPAPAEATGAPGEAPAPAAELGESAPSPKGSSSAALNGPTPGAPDDPIGLFVMRPQDGEVTVGGSITFSARVAGASLLKPPVVKWFKGKWVDLSSKVGQHLQLHDSYDRASKVYLFELHITDAQPAFTGSYRCEVSTKDKFDCSNFNLTVHEAMGTGDLDLLSAFRRTSLAGGGRRISDSHEDTGILDFSSLLKKRDSFRTPRDSKLEAPAEEDVWEILRQA

相同性解析による交差性が予測される生物種
Full Name myosin binding protein C, cardiac
Calculated molecular weight 1274 aa, 141 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC151211
Gene Symbol MYBPC3
Gene ID (NCBI) 4607
RRIDAB_2882814
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14896
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).

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