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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYBPC3 Polyclonal antibody (19977-1-AP)

    WB analysis using 19977-1-AP (same clone as 19977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using MYBPC3 Polyclonal antibody (19977-1-AP)

    WB analysis of human heart using 19977-1-AP (same clone as 19977-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using MYBPC3 Polyclonal antibody (19977-1-AP)

    WB analysis of rat heart using 19977-1-AP (same clone as 19977-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYBPC3 Polyclonal antibody (19977-1-AP)

    IHC staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYBPC3 Polyclonal antibody (19977-1-AP)

    IHC staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

MYBPC3 Polyclonal antibody, PBS Only

MYBPC3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19977-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Cardiac MyBP C, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC 3, Myosin-binding protein C, cardiac-type

Formulation: 
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19977-1-PBS targets MYBPC3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name myosin binding protein C, cardiac
Calculated molecular weight 141 kDa
Observed molecular weight 141-150 kDa
GenBank accession numberNM_000256
Gene Symbol MYBPC3
Gene ID (NCBI) 4607
RRIDAB_10858232
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14896
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249)

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