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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYBPC2 Polyclonal antibody (25257-1-AP)

    WB analysis using 25257-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25257-1-AP (MYBPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using MYBPC2 Polyclonal antibody (25257-1-AP)

    IP experiment of mouse skeletal muscle using 25257-1-AP

    IP result of anti-MYBPC2 (IP:25257-1-AP, 4ug; Detection:25257-1-AP 1:40000) with mouse skeletal muscle tissue lysate 1040 ug.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYBPC2 Polyclonal antibody (25257-1-AP)

    IF Staining of mouse skeletal muscle using 25257-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYBPC2 antibody (25257-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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MYBPC2 Polyclonal antibody

MYBPC2 Polyclonal Antibody for WB, IF-P, IP, ELISA
Cat No. 25257-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA

Synonyms

MYBPCF, Fast MyBP C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25257-1-AP targets MYBPC2 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYBPC2 fusion protein Ag19424 相同性解析による交差性が予測される生物種
Full Name myosin binding protein C, fast type
Calculated molecular weight 1141 aa, 128 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC136389
Gene Symbol MYBPC2
Gene ID (NCBI) 4606
RRIDAB_3669466
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14324
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYBPC2, also known as MYBPCF, fMyBP-C, is a member of the myosin-binding protein C (MYBPC)family. MYBPC is a myosin-associated protein found in the cross-bridge-bearing zone, or C region, of A bands in striated muscle. MYBPC has three paralogs encoded by unique genes, including slow skeletal (MYBPC1), fast skeletal (MYBPC2), and cardiac (MYBPC3). Slow skeletal MyBP-C (sMyBP-C), fast skeletal MyBP-C (fMyBP-C), and cardiac MyBP-C (cMyBP-C) proteins are highly conserved with over 90% homology. They play unique muscle-specific structural and regulatory roles in actomyosin interactions and contractility in striated muscles, including both cardiac and skeletal muscles (PMID: 35832193, 23936073).

Protocols

Product Specific Protocols
WB protocol for MYBPC2 antibody 25257-1-APDownload protocol
IF protocol for MYBPC2 antibody 25257-1-APDownload protocol
IP protocol for MYBPC2 antibody 25257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols