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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    WB analysis of mouse skeletal muscle using 22900-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    WB analysis of mouse skeletal muscle using 22900-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    WB analysis of mouse pancreas using 22900-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human skeletal muscle using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human skeletal muscle using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human heart using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human heart using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human bladder using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human bladder using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

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MYBPC1 Polyclonal antibody

MYBPC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22900-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MYBPC1, MYBPCC, MYBPCS, Slow MyBP C, slow type

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse pancreas tissue
Positive IHC detected inhuman skeletal muscle tissue, human bladder tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22900-1-AP targets MYBPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin binding protein C, slow type
Calculated molecular weight 128 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberNM_002465
Gene Symbol MYBPC1
Gene ID (NCBI) 4604
RRIDAB_11182592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00872
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYBPC1, also named as MYBPCS, belongs to the immunoglobulin superfamily and MyBP family. It is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. This antibody is specific to MYBPC1.

Protocols

Product Specific Protocols
WB protocol for MYBPC1 antibody 22900-1-APDownload protocol
IHC protocol for MYBPC1 antibody 22900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC

Mol Psychiatry

FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors.

Authors - Minjie Shen
View Article
ratWB

Dokl Biochem Biophys

Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Authors - I O Petrova
View Article
mouseIHC

Adv Biol (Weinh)

Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System

Authors - Mangesh Morey
View Article