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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    WB analysis of mouse skeletal muscle using 22900-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    WB analysis of mouse skeletal muscle using 22900-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    WB analysis of mouse pancreas using 22900-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of mouse skeletal muscle using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of mouse bladder using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of mouse heart using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human skeletal muscle using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human skeletal muscle using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human bladder using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human bladder tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human bladder using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human heart using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYBPC1 Polyclonal antibody (22900-1-AP)

    IHC staining of human heart using 22900-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

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MYBPC1 Polyclonal antibody

MYBPC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22900-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C-protein, skeletal muscle slow isoform, MYBPCS, Myosin-binding protein C, slow-type, Slow MyBP C, Slow MyBP-C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse pancreas tissue
Positive IHC detected inmouse skeletal muscle tissue, human bladder tissue, human heart tissue, human skeletal muscle tissue, mouse bladder tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22900-1-AP targets MYBPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin binding protein C, slow type
Calculated molecular weight 128 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberNM_002465
Gene Symbol MYBPC1
Gene ID (NCBI) 4604
RRIDAB_11182592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00872
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYBPC1, also named as MYBPCS, belongs to the immunoglobulin superfamily and MyBP family. It is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. This antibody is specific to MYBPC1.

Protocols

Product Specific Protocols
WB protocol for MYBPC1 antibody 22900-1-APDownload protocol
IHC protocol for MYBPC1 antibody 22900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIHC

Mol Psychiatry

FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors.

Authors - Minjie Shen
View Article
ratWB

Dokl Biochem Biophys

Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Authors - I O Petrova
View Article
mouseIHC

Adv Biol (Weinh)

Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System

Authors - Mangesh Morey
View Article