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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MXRA5 Polyclonal antibody

MXRA5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29777-1-AP

Synonyms

Adlican, DKFZp564I1922, MXRA5

Validation Data Gallery

Applications:
  • WB analysis using 29777-1-AP

    WB analysis using 29777-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 29777-1-AP (MXRA5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 29777-1-AP

    IHC staining of mouse liver using 29777-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29777-1-AP (MXRA5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 29777-1-AP

    IHC staining of mouse liver using 29777-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29777-1-AP (MXRA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-12 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29777-1-AP targets MXRA5 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MXRA5 fusion protein Ag31140 相同性解析による交差性が予測される生物種
Full Name matrix-remodelling associated 5
Calculated molecular weight 312 kDa
Observed molecular weight312 kDa
GenBank accession numberNM_015419
Gene symbol MXRA5
Gene ID (NCBI) 25878
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MXRA5 antibody 29777-1-APDownload protocol
IHC protocol for MXRA5 antibody 29777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols