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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MXRA5 Polyclonal antibody (25472-1-AP)

    WB analysis using 25472-1-AP (same clone as 25472-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25472-1-AP (MXRA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human kidney using 25472-1-AP (same clone as 25472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human colon cancer using 25472-1-AP (same clone as 25472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human colon cancer using 25472-1-AP (same clone as 25472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human kidney using 25472-1-AP (same clone as 25472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human liver using 25472-1-AP (same clone as 25472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human liver using 25472-1-AP (same clone as 25472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25472-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MXRA5 Polyclonal antibody, PBS Only

MXRA5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25472-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Adlican

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25472-1-PBS targets MXRA5 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name matrix-remodelling associated 5
Calculated molecular weight 312 kDa
Observed molecular weight163 kDa, 312 kDa
GenBank accession numberNM_015419
Gene Symbol MXRA5
Gene ID (NCBI) 25878
RRIDAB_2918088
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR99
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MXRA5, or adlican, is an adhesion proteoglycan involved in extracellular matrix (ECM) remodeling and cell-cell adhesion (PMID: 22696596). In kidney, MXRA5 has anti-inflammatory and anti-fibrotic properties by limiting the induction of chemokines, fibronectin and collagen expression in response to TGB1 and pro-inflammatory stimuli (PMID: 27599751). MXRA5 was identified as a novel biomarker in colorectal cancer, mainly located in the cytoplasm of CRC cells (PMID:23420087). Exome sequencing identified MXRA5 as a novel cancer gene mutated in non-small cell lung carcinoma (PMID: 22696596). MXRA5 was detected 163 kDa in human proximal tubular cells (PMID:27599751).

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