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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using MXI1 Recombinant antibody (84733-6-RR)

    IF Staining of SH-SY5Y using 84733-6-RR (same clone as 84733-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MXI1 antibody (84733-6-RR, Clone: 242089D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84733-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MXI1 Recombinant antibody (84733-6-RR)

    FC experiment of HeLa using 84733-6-RR (same clone as 84733-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MXI1 Recombinant antibody (84733-6-RR, Clone:242089D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84733-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84733-6-RR

    Affinity and Kinetic Characterization of 84733-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84733-6-RR against Human MXI1 were performed. The affinity constant is below 1 pM.

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MXI1 Recombinant antibody, PBS Only

MXI1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84733-6-PBS
Clone No.242089D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84733-6-PBS targets MXI1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MXI1 fusion protein Ag3024 相同性解析による交差性が予測される生物種
Full Name MAX interactor 1
Calculated molecular weight228 aa, 26 kDa
GenBank accession numberBC012907
Gene Symbol MXI1
Gene ID (NCBI) 4601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50539
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.