Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, SK-N-SH cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22583-1-AP targets MPDZ/MUPP1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18222 Product name: Recombinant human MUPP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 751-1100 aa of BC140793 Sequence: LMFVNDVNLENSSLEEAVEALKGAPSGTVRIGVAKPLPLSPEEGYVSAKEDSFLYPPHSCEEAGLADKPLFRADLALVGTNDADLVDESTFESPYSPENDSIYSTQASILSLHGSSCGDGLNYGSSLPSSPPKDVIENSCDPVLDLHMSLEELYTQNLLQRQDENTPSVDISMGPASGFTINDYTPANAIEQQYECENTIVWTESHLPSEVISSAELPSVLPDSAGKGSEYLLEQSSLACNAECVMLQNVSKESFERTINIAKGNSSLGMTVSANKDGLGMIVRSIIHGGAISRDGRIAIGDCILSINEESTISVTNAQARAMLRRHSLIGPDIKITYVPAEHLEEFKIS 相同性解析による交差性が予測される生物種 |
| Full Name | multiple PDZ domain protein |
| Calculated molecular weight | 2070 aa, 222 kDa |
| Observed molecular weight | 250 kDa |
| GenBank accession number | BC140793 |
| Gene Symbol | MPDZ |
| Gene ID (NCBI) | 8777 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75970 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PDZ domain is a protein motif that mediates protein-protein interactions. MUPP1(multi-PDZ domain protein-1) has 13 PDZ domains, each containing the structural characteristics of a prototypic PDZ motif, but lacks a catalytic domain. MUPP1 belongs to the PDZ domain protein family and is a structural homologant of PATJ (PALS1-associated tight-linking protein) (PMID: 23880463). MUPP1 is a scaffold protein and plays an important role in cytoskeletal organization, cell polarity, and cell proliferation(PMID: 25662616). Mutations in MUPP1 are associated with congenital hydrocephalus(PMID: 23240096).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MPDZ/MUPP1 antibody 22583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |