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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using MUC13 Recombinant monoclonal antibody (87107-2-RR)

    WB analysis of HT-29 using 87107-2-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 87107-2-RR (MUC13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using MUC13 Recombinant monoclonal antibody (87107-2-RR)

    IHC staining of human colon using 87107-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87107-2-RR (MUC13 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MUC13 Recombinant monoclonal antibody (87107-2-RR)

    IHC staining of human colon using 87107-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87107-2-RR (MUC13 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using MUC13 Recombinant monoclonal antibody (87107-2-RR)

    IF Staining of HT-29 using 87107-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using MUC13 antibody (87107-2-RR, Clone: 252211G3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87107-2-RR

    Affinity of 87107-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87107-1-RR against Human MUC13 were performed. The affinity constant is below 1 pM.

MUC13 Recombinant monoclonal antibody

MUC13 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 87107-2-RR
Clone No.252211G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Down-regulated in colon cancer 1, DRCC1, MUC-13, Mucin-13, RECC

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87107-2-RR targets MUC13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6586

Product name: Recombinant Human MUC13 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 19-421 aa of NM_033049.4

Sequence: TNQGNSADAVTTTETATSGPTVAAADTTETNFPETASTTANTPSFPTATSPAPPIISTHSSSTIPTPAPPIISTHSSSTIPIPTAADSESTTNVNSLATSDIITASSPNDGLITMVPSETQSNNEMSPTTEDNQSSGPPTGTALLETSTLNSTGPSNPCQDDPCADNSLCVKLHNTSFCLCLEGYYYNSSTCKKGKVFPGKISVTVSETFDPEEKHSMAYQDLHSEITSLFKDVFGTSVYGQTVILTVSTSLSPRSEMRADDKFVNVTIVTILAETTSDNEKTVTEKINKAIRSSSSNFLNYDLTLRCDYYGCNQTADDCLNGLACDCKSDLQRPNPQSPFCVASSLKCPDACNAQHKQCLIKKSGGAPECACVPGYQEDANGNCQKCAFGYSGLDCKDKFQL

相同性解析による交差性が予測される生物種
Full Name mucin 13, cell surface associated
Calculated molecular weight55 kDa
Observed molecular weight120 kDa
GenBank accession numberNM_033049.4
Gene Symbol MUC13
Gene ID (NCBI) 56667
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H3R2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mucins (MUC) are highly glycosylated proteins that are abundantly expressed in mammalian epithelial cells. Mucin 13 (MUC13) is a member of the transmembrane glycoprotein mucins, which are normally expressed in the large intestine, trachea, kidney, small intestine, and gastric epithelium (PMID: 22027689). MUC13 is involved in the tumorigenesis of multiple malignancies, including pancreatic cancer, colorectal cancer, renal cancer, and lung cancer (PMID: 22027689; PMID: 31427737; PMID: 28205224; PMID: 34966453).

Protocols

Product Specific Protocols
IF protocol for MUC13 antibody 87107-2-RRDownload protocol
IHC protocol for MUC13 antibody 87107-2-RRDownload protocol
WB protocol for MUC13 antibody 87107-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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