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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC13 Polyclonal antibody (55487-1-AP)

    WB analysis using 55487-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55487-1-AP (MUC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MUC13 Polyclonal antibody (55487-1-AP)

    IHC staining of human colon cancer using 55487-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 55487-1-AP (MUC13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MUC13 Polyclonal antibody

MUC13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55487-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Down-regulated in colon cancer 1, DRCC1, MUC-13, Mucin-13, RECC

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55487-1-AP targets MUC13 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name mucin 13, cell surface associated
Calculated molecular weight 55 kDa
Observed molecular weight90 kDa
GenBank accession numberNM_033049
Gene Symbol MUC13
Gene ID (NCBI) 56667
RRIDAB_3086455
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3R2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mucins (MUC) are highly glycosylated proteins that are abundantly expressed in mammalian epithelial cells. Mucin 13 (MUC13) is a member of the transmembrane glycoprotein mucins which is normally expressed in the large intestine, trachea, kidney, small intestine, and gastric epithelium (PMID: 22027689). MUC13 is involved in the tumorigenesis of multiple malignancies, including pancreatic cancer, colorectal cancer, renal cancer and lung cancer (PMID: 22027689; PMID: 31427737; PMID: 28205224; PMID: 34966453).

Protocols

Product Specific Protocols
IHC protocol for MUC13 antibody 55487-1-APDownload protocol
WB protocol for MUC13 antibody 55487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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